The GenePrint 24 System is a 24-locus multiplex system designed to generate a multi-locus human DNA profile from a variety of human-derived biological sources. These loci exhibit alleles that may differ in length between individuals and are inherited as codominant Mendelian traits. The term STR, also referred to as a microsatellite, relates to the number of base pairs of a tandemly repeated core DNA sequence which ranges from 2-8 base pairs in length. These loci consist of a core DNA sequence that is repeated a variable number of times within a discrete genetic locus. ^A buccal swab alternative source of DNA may only be used if there is an acceptable confidence level to determine the recipient’s genotype.Ĭhimerism testing (engraftment analysis) by DNA employs methodology commonly used in human identity testing and is accomplished by the analysis of genomic polymorphisms called short tandem repeat (STR) loci. Such samples will be processed however, a successful outcome may not be guaranteed. DNA specimens are only accepted if the nucleic acid isolation occurred in a CLIA-certified laboratory or a laboratory meeting equivalent requirements as determined by the CAP and/or the CMS. *DNA samples should be received only if they are the only samples available. Remarks: Post-transplantation results will be compared to pre-transplant recipient and donor genotypes, therefore, donor and recipient specimens should be obtained and genotyped before the transplant event occurs. Package and ship specimen cold, but not frozen. Specimen type: Whole blood, minimum 2 mL OR bone marrow, minimum 1 mL in lavender (EDTA) or yellow (ACD) collection tube OR DNA*. Package and ship specimen cold, but not frozen.Īlternatively: 2 buccal brushes^ each in a 2 mL tube with 650 uL of Lysis Buffer (only if engraftment has already occurred). Specimen type: Whole blood, minimum 2 mL in lavender (EDTA) or yellow (ACD) collection tube OR DNA*. Specimen type: Whole blood, minimum 2 mL in lavender (EDTA) or yellow (ACD) collection tube. The percent donor chimerism is calculated and reported, and a longitudinal plot of historic data is generated for successive post-transplant specimens.DNA is amplified via the Promega GenePrint24 System which employs methodology commonly used in human identity testing and is accomplished by the analysis of genomic polymorphisms called short tandem repeat (STR) loci. The DNA is isolated from the recipient, donor, and post-transplant samples.Next, after the transplant takes place, the performance of the transplant engraftment is assessed by evaluating the donor versus recipient contribution of white blood cells in post-transplant blood or bone marrow specimens obtained from the recipient. The test involves identifying the genetic profiles of the recipient and of the donor and then evaluating the extent of mixture in the recipient’s blood or bone marrow. First, DNA is isolated from the recipient and potential donor before the transplant and analysis is performed to determine whether the genetic markers unique to the donor and the recipient have sufficient power to distinguish the donor from the recipient. Chimerism testing (engraftment analysis) is performed for patients who have received a hematopoietic stem cell transplant.
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